tag:blogger.com,1999:blog-4989966954446423670.post4457597785041034083..comments2024-03-02T00:44:55.128-08:00Comments on Pleiotropy: Pleiotropy saves the day for evolving new genesBjørn Østmanhttp://www.blogger.com/profile/08859177313382114917noreply@blogger.comBlogger5125tag:blogger.com,1999:blog-4989966954446423670.post-79016312281219633292012-11-13T08:07:07.861-08:002012-11-13T08:07:07.861-08:00Thanks Richard.Thanks Richard.Bjørn Østmanhttps://www.blogger.com/profile/08859177313382114917noreply@blogger.comtag:blogger.com,1999:blog-4989966954446423670.post-31108429668228578962012-11-12T16:10:13.038-08:002012-11-12T16:10:13.038-08:00I threw you a trackback from my post pointing to y...I threw you a trackback from <a href="http://pandasthumb.org/archives/2012/11/evolving-a-new.html" rel="nofollow">my post pointing to yours</a>, but got an error. So let this serve in its stead.RBHhttps://www.blogger.com/profile/13562135000111792590noreply@blogger.comtag:blogger.com,1999:blog-4989966954446423670.post-12469992880435399962012-11-08T05:27:46.475-08:002012-11-08T05:27:46.475-08:00Interesting, I sort of expected them to duplicate ...Interesting, I sort of expected them to duplicate it themselves since I reasoned it would be unlikely to just wait for a specific gene to duplicate. <br /><br />But they seem to have done something in between engineering it and just letting it happen. Not that I think this alters anything about their conclusions of course, i was just curious about the methods used. Mikkel Rumraket Rasmussenhttps://www.blogger.com/profile/07670550711237457368noreply@blogger.comtag:blogger.com,1999:blog-4989966954446423670.post-76494632026389653032012-11-07T15:08:01.766-08:002012-11-07T15:08:01.766-08:00Here's what they say:
We placed this bifuncti...Here's what they say:<br /><br />We placed this bifunctional parental gene (dup13-15, D10G) under the control of a constitutive promoter that cotranscribed a yellow fluorescent protein ( yfp) gene. We also placed the T-his operon in a transposition-inactive transposable element Tn10d Tet close to the lac operon on the low–copy number (about two copies per chromosome) (11) F′128plasmid (Fig. 1C). Duplications and amplifications of this region are frequent and have low fitness cost (3), allowing experimen- tal study of the process within a reasonable time frame.<br /><br />Sounds like they waited for duplications.Bjørn Østmanhttps://www.blogger.com/profile/08859177313382114917noreply@blogger.comtag:blogger.com,1999:blog-4989966954446423670.post-91291664133662017662012-11-07T11:30:09.076-08:002012-11-07T11:30:09.076-08:00Interesting study. The paper is behind a paywall s...Interesting study. The paper is behind a paywall so I have a quick question. <br />Did they actually wait for the gene in question to duplicate in their experimental population, or did they edit the genes of the bacteria and then see what happened once a duplication occurred?Mikkel Rumraket Rasmussenhttps://www.blogger.com/profile/07670550711237457368noreply@blogger.com